| Sample |
PSI |
Passes |
Results |
Meningicoccal cell paste
(4%) in buffer |
1000 |
1 |
Cooling used to maintain 10C temperature. Complete rupture achieved in one pass. |
| Mammalian cells |
2000 |
1 |
Complete rupture of cells and easy separation of parasite within the cell achieved in one pass on laboratory model H-5000. |
| E. coli (10%) in buffer |
6000 |
3 |
90% rupture achieved. Results scaled linearly from lab to production. |
|
10000 |
1 |
90% rupture achieved. |
|
10000 |
3 |
90% rupture achieved. |
| Baker's yeast |
20000 |
10 |
Cooling used. 95% rupture of yeast cells achieved. |
| Brewer's yeast (10%) in water |
10000 |
1-10 |
Cooling used to keep temp below 5C. 35% rupture at 1 pass and 63% in 10 passes. |
|
20000 |
1-10 |
71% rupture at 1 pass and 98% in 10 passes. Yield of enzyme significantly better than that achieved with other processing techniques including French press. |
Processors
